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The reaction surface area research variety, a CCD with 11 operates, was applied. The proposed CCD experimental structure was used as well as the evaluation of cellular stage composition and pH of buffer was executed in opposition to the three responses, retention time, peak place, and symmetry component; the effects are summarized in Table two and
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For agar media, have a loopful of organism and streak it on surface area of media or carry out spread plate with 0.1ml of inoculum obtaining a lot less than 100CFU of microorganism from society suspension and incubate it at acceptable temperature and time frame mentioned in Table-1.Growth promotion test (GPT): Also generally known as fertility or n
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